TRAF6 Recombinant Rabbit Monoclonal Antibody [JE01-42]
cat.: HA722857
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE01-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | K-562 cell lysate, 293T cell lysate, HeLa cell lysate, COS-1 cell lysate, CTLL-2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, PC-12, HeLa, RAW264.7. |
| Subcellular location: | Cytoplasm, cell cortex, Nucleus, Lipid droplet. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9Y4K3 Human | P70196 Mouse | B5DF45 Rat |
| Alternative names: | E3 ubiquitin-protein ligase TRAF6 Interleukin 1 signal transducer Interleukin-1 signal transducer MGC 3310 MGC:3310 MGC3310 OTTHUMP00000232772 OTTHUMP00000232773 RING finger protein 85 RNF 85 RNF85 TNF receptor associated factor 6 TNF receptor-associated factor 6 TNF receptor-associated factor 6, E3 ubiquitin protein ligase TRAF 6 Traf6 TRAF6_HUMAN |
Images
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Fig1:
Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (HA722857) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: CTLL-2 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722857) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling TRAF6 with Rabbit anti-TRAF6 antibody (HA722857) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRAF6 antibody (HA722857) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling TRAF6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722857, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Flow cytometric analysis of RAW264.7 cells labeling TRAF6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722857, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of PC-12 cells labeling TRAF6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722857, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.