KLRG1 Recombinant Rabbit Monoclonal Antibody [JE33-71]
cat.: HA722859
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human KLRG1 aa 1-38 / 195. |
| Positive control: | NK-92 cell lysate, Jurkat cell lysate, MoLT-4 cell lysate, EL4 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q96E93 Human | O88713 Mouse | Q64335 Rat |
| Alternative names: | 2F1 Ag 2F1 C type lectin domain family 15 member A C-type lectin domain family 15 member A CLEC15A ITIM containing receptor MAFA L ITIM-containing receptor MAFA-L Killer cell lectin like receptor G1 Killer cell lectin like receptor subfamily G member 1 Killer cell lectin-like receptor subfamily G member 1 KLRG 1 KLRG1 KLRG1 protein KLRG1_HUMAN MAFA 2F1 MAFA MAFA L MAFA like MAFA like receptor MAFA-like receptor MAFAL Mast cell function associated antigen (ITIM containing) Mast cell function associated antigen Mast cell function-associated antigen 2F1 Mast cell function-associated antigen Mast cell function-associated antigen, rat, homolog of MGC13600 |
Images
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Fig1:
Western blot analysis of KLRG1 on different lysates with Rabbit anti-KLRG1 antibody (HA722859) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: Jurkat cell lysate Lane 3: MoLT-4 cell lysate Lane 4: EL4 cell lysate Lane 5: mouse spleen tissue lysate Lane 6: rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 35 kDa,75 kDa Exposure time: 1 minutes 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.