smooth muscle Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody [JE60-21]
cat.: HA722863
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 227 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human smooth muscle Myosin heavy chain 11 aa 1-50 / 1,972. |
| Positive control: | Mouse small intestine tissue lysate, rat ovary tissue lysate, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue. |
| Subcellular location: | Melanosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P35749 Human | O08638 Mouse | Q63862 Rat |
| Alternative names: | AAT4 DKFZp686D10126 DKFZp686D19237 FAA4 FLJ35232 MGC126726 MGC32963 MYH 11 MYH11 MYH11_HUMAN Myosin 11 Myosin heavy chain 11 Myosin heavy chain 11 smooth muscle Myosin heavy chain Myosin heavy chain smooth muscle isoform Myosin heavy polypeptide 11 smooth muscle Myosin-11 SMHC SMMHC smooth muscle isoform Smooth muscle myosin heavy chain 11 isoform SM2 Smooth muscle myosin heavy chain isoform SM2 |
Images
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Fig1:
Western blot analysis of smooth muscle Myosin heavy chain 11 on different lysates with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/1,000 dilution. Lane 1: mouse small intestine tissue lysate (40 µg/Lane) Lane 2: rat ovary tissue lysate (40 µg/Lane) Predicted band size: 227 kDa Observed band size: 227 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722863) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.