Connexin 32 / GJB1 Recombinant Rabbit Monoclonal Antibody [JE54-70]
cat.: HA722869
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Connexin 32 / GJB1 aa 46-75 / 283. |
| Positive control: | MCF7 cell lysate, 293T cell lysate, Human lung tissue lysate, Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Cell membrane, Cell junction, gap junction. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P08034 Human | P28230 Mouse | P08033 Rat |
| Alternative names: | Charcot Marie Tooth neuropathy X linked CMTX 1 CMTX CMTX1 Connexin-32 Connexin32 CX 32 Cx32 CXB1_HUMAN GAP junction 28 kDa liver protein Gap junction beta 1 protein Gap junction beta-1 protein Gap junction protein beta 1 32kD Gap junction protein beta 1 GJB 1 GJB1 |
Images
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Fig1:
Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (HA722869) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Human lung tissue lysate (40 µg/Lane) Lane 4: Human liver tissue lysate (40 µg/Lane) Predicted band size: 32 kDa Observed band size: 26 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (HA722869) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate (negative) Lane 3: Rat liver tissue lysate Lane 4: Rat spleen tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 32 kDa Observed band size: 48 kDa Exposure time: 1 minute 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722869) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.