Dynamin 3 Recombinant Rabbit Monoclonal Antibody [JE54-86]
cat.: HA722870
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: JE54-86
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 98 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Dynamin 3 aa 750-850.
Positive control: Human brain tissue lysate, Mouse brain tissue lysate.
Subcellular location: Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9UQ16 Human | Q8BZ98 Mouse
Alternative names: testicular dynamin testicular DNM 3 DNM3 DNM3 protein Dyn3 DYN3_HUMAN Dyna III DynaIII dynamin 2 Dynamin Dynamin family member Dynamin testicular Dynamin-3 Dynamin3 EC 3.6.5.5 KIAA0820 MGC70433 T dynamin T-dynamin
Images
HA722870_1.jpg Fig1: Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA722870) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 98 kDa
Observed band size: 100 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722870) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.