| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Dynamin 3 aa 750-850. |
| Positive control: | Human brain tissue lysate, Mouse brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9UQ16 Human | Q8BZ98 Mouse |
| Alternative names: | testicular dynamin testicular DNM 3 DNM3 DNM3 protein Dyn3 DYN3_HUMAN Dyna III DynaIII dynamin 2 Dynamin Dynamin family member Dynamin testicular Dynamin-3 Dynamin3 EC 3.6.5.5 KIAA0820 MGC70433 T dynamin T-dynamin |
|
Fig1:
Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA722870) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722870) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |