Human IL-13R alpha 2 Recombinant Rabbit Monoclonal Antibody [PSH07-64]
cat.: HA722881
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-13R alpha 2 aa 27-343. |
| Positive control: | Rrecombinant Human IL-13R alpha 2 protein (HA210969). |
| Subcellular location: | Extracellular. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-63] to Human IL-13R alpha 2 antibody (Capture) (HA722880) and recombinant Human IL-13R alpha 2 protein (HA210969) as the standard. The reference range value is 0.247-20 ng/ml. |
| Uniprot #: | SwissProt: Q14627 Human |
| Alternative names: | Cancer/testis antigen 19 CD213a2 CT19 I13R2_HUMAN IL-13 receptor subunit alpha-2 IL-13R subunit alpha-2 IL-13R-alpha-2 IL-13RA2 IL13 receptor IL13BP IL13R Il13ra2 Interleukin 13 binding protein Interleukin 13 receptor alpha 2 chain Interleukin-13 receptor subunit alpha-2 Interleukin-13-binding protein |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-13 R alpha 2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722880) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rrecombinant Human IL-13R alpha 2 protein (HA210969) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA722881, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native human IL-13 R alpha 2 in U-87 MG extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 50%. The mean human IL-13 R alpha 2 concentration was determined to be 4,569 pg/ml in U-87 MG extract. |
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Fig3:
Interpolated concentrations of native human IL-13 R alpha 2 in A375 extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are A375 extract 10%. The mean human IL-13 R alpha 2 concentration was determined to be 123.1 ng/ml in A375 extract. |
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Fig4:
Interpolated concentrations of spiked IL-13 R alpha 2 in human cell culture media samples. The concentrations of IL-13 R alpha 2 were interpolated from the IL-13 R alpha 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.