GWL Recombinant Rabbit Monoclonal Antibody [PSH07-69]
cat.: HA722884
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH07-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GWL aa 1-350. |
| Positive control: | HeLa cell lysate, LNCaP cell lysate, HepG2 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus, Cleavage furrow. |
| Recommended Dilutions:
WB IF-Cell IP |
1:2,000 1:50 1-2μg/sample |
| Uniprot #: | SwissProt: Q96GX5 Human |
| Alternative names: | 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L Mastl MGC117975 Microtubule associated serine/threonine kinase like Microtubule-associated serine/threonine-protein kinase-like RP11 85G18.2 Serine/threonine-protein kinase greatwall THC2 |
Images
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Fig1:
Western blot analysis of GWL on different lysates with Rabbit anti-GWL antibody (HA722884) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: LNCaP cell lysate Lane 3: HepG2 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 110 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722884) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling GWL with Rabbit anti-GWL antibody (HA722884) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GWL antibody (HA722884) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/50 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
GWL was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722884 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722884 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722884 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722884 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.