Nephrin Recombinant Rabbit Multiclonal Antibody [PSH07-70]
cat.: HA722886
| Product Type: | Recombinant Rabbit multiclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr |
| Clone number: | PSH07-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 135 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Nephrin aa 1-1,050. |
| Positive control: | Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr |
1:1,000-1:5,000 1:3,000 1:200-1:1,000 1:500 |
| Uniprot #: | SwissProt: O60500 Human | Q9QZS7 Mouse | Q9R044 Rat |
| Alternative names: | CNF Nephrin Nephrosis 1 congenital Finnish type Nephrosis 1, congenital, Finnish type (nephrin) NPHN NPHN_HUMAN NPHS 1 Nphs1 Renal glomerulus specific cell adhesion receptor Renal glomerulus-specific cell adhesion receptor |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/500 dilution. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722886, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Western blot analysis of Nephrin on different lysates with Rabbit anti-Nephrin antibody (HA722886) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse spleen tissue lysate (negative) Lane 3: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 135 kDa Observed band size: 200 kDa Exposure time: 1 minute 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722886) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722886) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722886) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Nephrin antibody (HA722886) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722886) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of Nephrin on different lysates with Rabbit anti-Nephrin antibody (HA722886) at 1/5,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse kidney tissue lysate treated with deglycosylation Lysates/proteins at 14.4 µg/Lane. Predicted band size: 135 kDa Observed band size: 200/135 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722886) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.