TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]
cat.: HA722891
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIM 3 aa 1-223. |
| Positive control: | RPMI 8226 cell lysate, HUT 102 cell lysate. |
| Subcellular location: | Membrane, Cell junction, Cell membrane. |
| Recommended Dilutions:
WB |
1:4,000 |
| Uniprot #: | SwissProt: Q8TDQ0 Human |
| Alternative names: | CD366 FLJ14428 HAVcr-2 Havcr2 HAVR2_HUMAN Hepatitis A virus cellular receptor 2 Kidney injury molecule 3 KIM 3 KIM3 T cell immunoglobulin and mucin domain containing 3 T cell immunoglobulin mucin 3 T-cell immunoglobulin and mucin domain-containing protein 3 T-cell immunoglobulin mucin family member 3 T-cell immunoglobulin mucin receptor 3 T-cell membrane protein 3 Tim 3 TIM-3 TIM3 TIMD-3 TIMD3 |
Images
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Fig1:
Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (HA722891) at 1/4,000 dilution. Lane 1: RPMI 8226 cell lysate (20 µg/Lane) Lane 2: HUT 102 cell lysate (40 µg/Lane) Lane 3: HT-29 cell lysate (negative) (40 µg/Lane) Predicted band size: 33 kDa Observed band size: 55 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722891) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.