CD79a Recombinant Rabbit Monoclonal Antibody [JE32-14]
cat.: HA722896
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE32-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD79a aa 51-100 / 226. |
| Positive control: | Ramos cell lysate, Daudi cell lysate, Raji cell lysate, Daudi, human colon tissue, human lung cancer tissue, human spleenr tissue. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:1,000 1:50 1:200-1:1,000 1:50 |
| Uniprot #: | SwissProt: P11912 Human |
| Alternative names: | B lymphocyte-specific MB1 protein B-cell antigen receptor complex-associated protein alpha chain CD 79a CD79a CD79a antigen (immunoglobulin-associated alpha) CD79A antigen CD79a molecule, immunoglobulin-associated alpha CD79A_HUMAN Ig alpha Ig-alpha IGA IgM-alpha Immunoglobulin-associated alpha Ly54 MB-1 membrane glycoprotein MB1 Membrane-bound immunoglobulin-associated protein Surface IgM-associated protein |
Images
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Fig1:
Western blot analysis of CD79a on different lysates with Rabbit anti-CD79a antibody (HA722896) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Daudi cell lysate Lane 3: Raji cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 45-55 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722896) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Daudi cells labeling CD79a with Rabbit anti-CD79a antibody (HA722896) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD79a antibody (HA722896) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD79a antibody (HA722896) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722896) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-CD79a antibody (HA722896) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleenr tissue with Rabbit anti-CD79a antibody (HA722896) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/50 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.