| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GABRB1 aa 351-400 / 474. |
| Positive control: | Human brain tissue lysate, Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P18505 Human | P50571 Mouse | P15431 Rat |
| Alternative names: | AW061132 B230208N19Rik GABA(A) receptor beta 1 GABA(A) receptor subunit beta-1 GABA-A receptor, beta-1 polypeptide Gabrb-1 GABRB1 Gamma aminobutyric acid (GABA) A receptor beta 1 Gamma Aminobutyric Acid A Receptor Beta 1 Gamma Aminobutyric Acid Receptor , beta-1 Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 Gamma-aminobutyric acid receptor subunit beta-1 GARB1 GBRB1_HUMAN |
|
Fig1:
Western blot analysis of GABRB1 on different lysates with Rabbit anti-GABRB1 antibody (HA722898) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat cerebellum tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722898) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |