TEMT Recombinant Rabbit Monoclonal Antibody [JE64-74]
cat.: HA722900
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | A549 cell lysate, Mouse liver tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate, human lung tissue, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O95050 Human | P40936 Mouse Entrez Gene: 368066 Rat |
| Alternative names: | Amine N methyltransferase Amine N-methyltransferase Aromatic alkylamine N methyltransferase Aromatic alkylamine N-methyltransferase Arylamine N methyltransferase Arylamine N-methyltransferase Indolamine N-methyltransferase Indolethylamine N methyltransferase Indolethylamine N-methyltransferase Inmt INMT_HUMAN nicotine N methyltransferase TEMT thioester S methyltransferase like Thioether S methyltransferase |
Images
|
Fig1:
Western blot analysis of TEMT on different lysates with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Rat lung tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: Lane 1: 3 minutes; Lane 2-5: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.