Human Beta Nerve Growth Factor Recombinant Rabbit Monoclonal Antibody [PSH07-81]
cat.: HA722904
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Beta Nerve Growth Factor aa 19-241 (HA211342). |
| Positive control: | Recombinant Human Beta Nerve Growth Factor protein (HA211342). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-79] to Human Beta Nerve Growth Factor antibody (Capture) (HA722902) or Rabbit monoclonal [PSH07-80] to Human Beta Nerve Growth Factor antibody (Capture) (HA722903) and recombinant Human Human Beta Nerve Growth Factor protein (HA211342) as the standard. The reference range value is 10.3-2,500pg/ml. |
| Uniprot #: | SwissProt: P01138 Human |
| Alternative names: | Beta nerve growth factor Beta NGF Beta-nerve growth factor Beta-NGF HSAN5 MGC161426 MGC161428 Nerve growth factor (beta polypeptide) Nerve growth factor Nerve growth factor beta Nerve growth factor beta polypeptide Nerve growth factor beta subunit NGF NGF_HUMAN NGFB NID67 |
Images
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Fig1:
Sandwich ELISA analysis of human beta-NGF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722903) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Beta Nerve Growth Factor protein (HA211342) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA722904, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of human beta-NGF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722902) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Beta Nerve Growth Factor protein (HA211342) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA722904, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of spiked beta-NGF in human cell culture media samples. The concentrations of beta-NGF were measured in duplicates, interpolated from the beta-NGF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig4:
Interpolated concentrations of spiked beta-NGF in human cell culture media samples. The concentrations of beta-NGF were measured in duplicates, interpolated from the beta-NGF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.