Human CD31 Recombinant Rabbit Monoclonal Antibody [PSH07-82]
cat.: HA722906
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD31 aa 28-601 (HA210891). |
| Positive control: | Recombinant Human CD31 protein (HA210891). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-83] to Human CD31 antibody (Detector) (HA722906) and recombinant Human CD31 protein as the standard (HA210891). The reference range value is 82.3-20,000pg/ml. |
| Uniprot #: | SwissProt: P16284 Human |
| Alternative names: | Adhesion molecule CD31 CD31 antigen CD31 EndoCAM EndoCAM FLJ34100 FLJ58394 GPIIA GPIIA' PECA1 PECA1_HUMAN Pecam 1 PECAM 1 CD31 EndoCAM PECAM PECAM-1 Pecam1 Platelet and endothelial cell adhesion molecule 1 Platelet endothelial cell adhesion molecule Platelet/endothelial cell adhesion molecule 1 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD31 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722906) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD31 protein (HA210891) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA722907, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD31in human serum samples. The concentrations of CD31 were interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are human serum 20%. The mean CD31 concentration was determined to be 39.7 ng/mL in human serum. |
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Fig3:
Interpolated concentrations of native CD31 in THP-1 and HeLa extract samples based on a 1,000 µg/ml extract load. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 3% and HeLa extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 68.19 ng/ml in THP-1 extract and undetectable in HeLa extract. |
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Fig4:
Interpolated concentrations of spiked CD31 in human cell culture media samples. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.