SMOC1 Recombinant Rabbit Monoclonal Antibody [PSH07-84]
cat.: HA722909
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SMOC1 aa 1-434. |
| Positive control: | HeLa cell lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9H4F8 Human |
| Alternative names: | Secreted modular calcium-binding protein 1 SMOC-1 SMOC1 SMOC1_HUMAN SPARC-related modular calcium-binding protein 1 |
Images
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Fig1:
Western blot analysis of SMOC1 on different lysates with Rabbit anti-SMOC1 antibody (HA722909) at 1/2,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si SMOC1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722909) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.