Biotin Conjugated 5-HT (Serotonin) Recombinant Rabbit Monoclonal Antibody [PSH06-04]
cat.: HA722914
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH06-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | 5-HT-OVA |
| Alternative names: | 5 hydroxytryptamine 5HT |
Images
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Fig1: Indirect ELISA analysis of 5-HT was performed by coating wells of a 96-well plate with 50 µL per well of 5-HT-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 50 µL per well of 5-HT-Biotin monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 min at 37℃. The plate was washed and incubated with 50 µL per well of an SA-HRP-conjugated goat rabbit IgG secondary antibody at a dilution of 1:2,000 for 30 min at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.