MEK6 Recombinant Rabbit Monoclonal Antibody [PSH07-86]
cat.: HA722917
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: PSH07-86
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MEK6 aa 1-50.
Positive control: HeLa cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.
Subcellular location: Nucleus, Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB
  IP

1:2,000
1-2μg/sample
Uniprot #: SwissProt: P52564 Human | P70236 Mouse
Entrez Gene: 114495 Rat
Alternative names: Dual specificity mitogen activated protein kinase kinase 6 Dual specificity mitogen-activated protein kinase kinase 6 MAP kinase kinase 6 MAP2K6 MAPK/ERK kinase 6 MAPKK 6 MAPKK6 MEK 6 MEK6 Mitogen Activated Protein Kinase Kinase 6 MKK 6 MKK6 MP2K6_HUMAN PRKMK6 protein kinase Protein kinase mitogen activated kinase 6 protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) SAPK kinase 3 SAPKK-3 SAPKK3 SKK3 Stress-activated protein kinase kinase 3
Images
HA722917_1.jpg Fig1: Western blot analysis of MEK6 on different lysates with Rabbit anti-MEK6 antibody (HA722917) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (30 µg/Lane)
Lane 7: Rat skeletal muscle tissue lysate (30 µg/Lane)

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: Lane 1-5: 2 minutes 7 seconds; Lane 6-7: 18 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722917) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.