TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]
cat.: HA722918
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH07-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TBR2 aa 1-300. |
| Positive control: | NK-92 cell lysate, Mouse embryo tissue lysate, E14.5 mouse embryonic brain tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:1,000 1:5,000 1:500 |
| Uniprot #: | SwissProt: O95936 Human | O54839 Mouse |
| Alternative names: | eomes EOMES_HUMAN Eomesodermin Eomesodermin homolog t box brain 2 T-box brain protein 2 T-brain-2 Tbr 2 TBR-2 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: E14.5 embryonic brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (HA722918) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722918) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of TBR2 on different lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 73/85 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Western blot analysis of TBR2 on mouse embryo tissue lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 73/85 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.