ATP5G1 Recombinant Rabbit Monoclonal Antibody [JE64-60]
cat.: HA722921
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ATP5G1 aa 51-100 / 136. |
| Positive control: | HeLa cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, rat small intestine tissue. |
| Subcellular location: | Mitochondrion membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: P05496 Human | Q9CR84 Mouse | Q06645 Rat |
| Alternative names: | ATP synthase lipid-binding protein ATP synthase membrane subunit c locus 1 Imported ATP synthase proteolipid P1 ATP synthase proton-transporting mitochondrial F(0) complex subunit C1 ATPase protein 9 ATPase subunit c |
Images
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Fig1:
Western blot analysis of ATP5G1 on different lysates with Rabbit anti-ATP5G1 antibody (HA722921) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Human liver tissue lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (10 µg/Lane) Lane 5: Rat liver tissue lysate (10 µg/Lane) Predicted band size: 14 kDa Observed band size: 8 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722921) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ATP5G1 antibody (HA722921) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722921) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.