Mouse RANKL Recombinant Rabbit Monoclonal Antibody [PSH07-90]
cat.: HA722922
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse RANKL aa 158-316 (HA210558). |
| Positive control: | Recombinant Mouse RANKL protein (HA210558). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-92] to Mouse RANKL antibody (Detector) (HA722924) and recombinant Mouse RANKL protein (HA210558) as the standard. The reference range value is 8.2-2,000pg/ml. |
| Uniprot #: | SwissProt: O35235 Mouse |
| Alternative names: | CD254 hRANKL2 ODF OPGL OPTB2 Osteoclast differentiation factor Osteoprotegerin ligand Rank Ligand RANKL Receptor activator of nuclear factor kappa B ligand Receptor activator of nuclear factor kappa-B ligand sOdf TNF related activation induced cytokine TNF-related activation-induced cytokine TNF11_HUMAN TNFSF 11 Tnfsf11 TRANCE Tumor necrosis factor (ligand) superfamily member 11 Tumor necrosis factor ligand superfamily member 11 Tumor necrosis factor ligand superfamily member 11, soluble form |
Images
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Fig1:
Sandwich ELISA analysis of mouse RANKL matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722922) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse RANKL protein (HA210558) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722924, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native RANKL in mouse spleen cell culture supernatant treated or untreated with concanavalin A for 3days . Interpolated concentration of native RANKL was measured in duplicate at different sample concentrations and interpolated from the RANKL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean RANKL concentration was determined to be 3,251 pg/mL in neat mouse spleen cell treated supernatant, undetectable in untreated mouse spleen cell supernatant. |
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Fig3:
Interpolated concentrations of spiked RANKL in human cell culture media samples. The concentrations of RANKL were measured in duplicates, interpolated from the RANKL standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.