Human MMP-7 Recombinant Rabbit Monoclonal Antibody [PSH07-94]
cat.: HA722927
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP-7 aa 18-267 (HA210989). |
| Positive control: | Recombinant Human MMP-7 protein (HA210989) |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-93] to Human MMP-7 antibody (Capture) (HA722926) and recombinant Human MMP-7 protein (HA210989) as the standard. The reference range value is 32.9-8,000 pg/ml. |
| Uniprot #: | SwissProt: P09237 Human |
| Alternative names: | Matrilysin Matrin Matrix Metalloproteinase 7 Matrix metalloproteinase-7 MMP 7 MMP-7 MMP7 MMP7_HUMAN MPSL1 PUMP 1 Pump 1 protease Pump-1 protease PUMP1 Uterine matrilysin Uterine metalloproteinase |
Images
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Fig1:
Sandwich ELISA analysis of Human MMP-7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-7 protein (HA210989) starting from 20000 pg/ml to 0 pg/ml and detect antibody (HA722927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native total MMP7 in human cell culture supernatant samples. Interpolated concentration of native total MMP-7 was measured in duplicate at different sample concentrations and interpolated from the total MMP-7 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean total MMP-7 concentration was determined to be 454,056 pg/mL in HT-29 and 1287 pg/ml in A549 in cell culture supernatant, undetectable in LNCaP supernatant. |
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Fig3:
Interpolated concentrations of spiked Pro-MMP-7 in cell culture media samples. The concentrations of Pro-MMP-7 were measured in duplicates, interpolated from thel Pro-MMP-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.