DNAJC6 Recombinant Rabbit Monoclonal Antibody [PSH07-95]
cat.: HA722929
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH07-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DNAJC6 aa 714-913. |
| Positive control: | Human brain tissue lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasmic vesicle, clathrin-coated vesicle. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:2,000-1:5,000 1:200-1:1,000 1:200-1:500 1:200-1:500 |
| Uniprot #: | SwissProt: O75061 Human | Q80TZ3 Mouse Entrez Gene: 313409 Rat |
| Alternative names: | auxilin DJC6 DnaJ (Hsp40) homolog, subfamily B, member 6 DnaJ (Hsp40) homolog, subfamily C, member 6 DnaJ homolog subfamily C member 6 EC 3.1.3.48 KIAA0473 MGC129914 MGC129915 MGC48436 putative tyrosine-protein phosphatase auxilin |
Images
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Fig1:
Western blot analysis of DNAJC6 on different lysates with Rabbit anti-DNAJC6 antibody (HA722929) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human liver tissue lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse cerebellum tissue lysate Lane 5: Mouse liver tissue lysate (negative) Lane 6: Rat brain tissue lysate Lane 7: Rat liver tissue lysate (negative) Lane 8: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 110 kDa Exposure time: Lane 1-8 (left): 30 seconds; Lane 1-8 (right): 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722929) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DNAJC6 antibody (HA722929) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722929) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-DNAJC6 antibody (HA722929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DNAJC6 antibody (HA722929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-DNAJC6 antibody (HA722929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-DNAJC6 antibody (HA722929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-DNAJC6 antibody (HA722929) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig9:
Application: IHC-Fr Species: Mouse Site: Liver (negative) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig10:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.