Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [JE40-30]
cat.: HA722930
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Desmoglein 3 aa 901-950 / 999. |
| Positive control: | HaCaT cell lysate, A431 cell lysate, human esophagus tissue, mouse esophagus tissue, rat esophagus tissue. |
| Subcellular location: | Cell membrane Curated, Cell junction, desmosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P32926 Human | O35902 Mouse Entrez Gene: 291752 Rat |
| Alternative names: | 130 kD pemphigus vulgaris antigen 130 kDa pemphigus vulgaris antigen Balding Cadherin family member 6 CDHF 6 CDHF6 Desmoglein 3 Desmoglein 3 precursor Desmoglein-3 Desmoglein3 DKFZp686P23184 DSG 3 DSG3 DSG3_HUMAN Pemphigus vulgaris antigen PVA |
Images
|
Fig1:
Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution. Lane 1: HaCaT cell lysate Lane 2: A431 cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 140 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722930) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722930) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722930) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722930) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.