Mouse Pro-Collagen I alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH07-98]
cat.: HA722934
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Pro-Collagen I alpha 1 protein aa aa 23-266 and 1083-1453. |
| Positive control: | Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-99] to Mouse Pro-Collagen I alpha 1 antibody (Detector) (HA722935) and recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) as the standard. The reference range value is 32.9-8,000 pg/ml. |
| Uniprot #: | SwissProt: P11087 Mouse |
| Alternative names: | Alpha 1 type I collagen Alpha-1 type I collagen alpha1(I) procollagen CO1A1_HUMAN COL1A1 collagen 1 collagen alpha 1 chain type I Collagen alpha-1(I) chain collagen alpha-1(I) chain preproprotein Collagen I alpha 1 polypeptide collagen of skin, tendon and bone, alpha-1 chain Collagen type I alpha 1 EDSC OI1 OI2 OI3 OI4 pro-alpha-1 collagen type 1 type I proalpha 1 Type I procollagen type I procollagen alpha 1 chain |
Images
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Fig1:
Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722935, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 2146 ng/mL in mouse serum, 170 ng/ml in NIH/3T3 and 10 ng/ml mouse lung cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked Pro‑Collagen I alpha 1 in cell culture media samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] The concentrations of Pro‑Collagen I alpha 1 were measured in duplicates, interpolated from the Pro‑Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.