HRP Conjugated Mouse Pro-Collagen I alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH07-99]
cat.: HA722936H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Pro-Collagen I alpha 1 protein aa aa 23-266 and 1083-1453. |
| Positive control: | Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-98] to Mouse Pro-Collagen I alpha 1 antibody (Capture) (HA722934) and recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) as the standard. The reference range value is 312.5-40,000 pg/ml. |
| Uniprot #: | SwissProt: P11087 Mouse |
| Alternative names: | Alpha 1 type I collagen Alpha-1 type I collagen alpha1(I) procollagen CO1A1_HUMAN COL1A1 collagen 1 collagen alpha 1 chain type I Collagen alpha-1(I) chain collagen alpha-1(I) chain preproprotein Collagen I alpha 1 polypeptide collagen of skin, tendon and bone, alpha-1 chain Collagen type I alpha 1 EDSC OI1 OI2 OI3 OI4 pro-alpha-1 collagen type 1 type I proalpha 1 Type I procollagen type I procollagen alpha 1 chain |
Images
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Fig1:
Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA722935, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.