Phospho-Nucleolin (T76) Recombinant Rabbit Monoclonal Antibody [JE64-03]
cat.: HA722942
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr76 of Human Nucleolin. |
| Positive control: | K-562 cell lysate, K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, human tonsil tissue, human kidney cancer tissue. |
| Subcellular location: | Nucleus, nucleolus;Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P19338 Human |
| Alternative names: | C23 FLJ45706 MS1116 NCL Nucl NUCL_HUMAN Nucleolin Protein C23 |
Images
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Fig1:
Western blot analysis of Phospho-Nucleolin (T76) on different lysates with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate (20 µg/Lane) Lane 3: K-562 treated with 50ng/mL Calyculin A for 20 minutes cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane) Predicted band size: 77 kDa Observed band size: 105 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722942) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722942) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue with Rabbit anti-Phospho-Nucleolin (T76) antibody (HA722942) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722942) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.