Human TRAIL Recombinant Rabbit Monoclonal Antibody [PSH08-06]
cat.: HA722944
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: ELISA(Det)
Clonality: Monoclonal
Clone number: PSH08-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Human TRAIL aa 114-281.
Positive control: Recombinant Human TRAIL protein as the standard (HA210893).
Subcellular location: Secreted.
Recommended Dilutions:
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-05] to Human TRAIL antibody (Capture) (HA722943) and recombinant Human TRAIL protein as the standard (HA210893). The reference range value is 16.5-4,000 pg/ml.
Uniprot #: SwissProt: P50591 Human
Alternative names: Apo 2 ligand APO 2L Apo-2 ligand Apo-2L APO2L CD253 CD253 antigen Chemokine tumor necrosis factor ligand superfamily member 10 Protein TRAIL TL2 TNF Related Apoptosis Inducing Ligand TNF related apoptosis inducing ligand TRAIL TNF-related apoptosis-inducing ligand TNF10_HUMAN TNFSF10 TRAIL Tumor necrosis factor (ligand) family member 10 Tumor Necrosis Factor (ligand) Superfamily Member 10 Tumor necrosis factor apoptosis inducing ligand splice variant delta Tumor necrosis factor ligand superfamily member 10
Images
HA722944_1.png Fig1: Sandwich ELISA analysis of human TRAIL matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722943) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAIL protein as the standard (HA210893) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722944, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA722944_2.png Fig2: Interpolated concentrations of native TRAIL in 786-0 and Hela extract samples based on a 1000 µg/ml extract load.

Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 11,821 pg/mL in 786-0 cell extract, There was no detectable signal in Hela cell extract.
HA722944_3.png Fig3: Interpolated concentrations of native TRAIL in cell culture supernatant samples.

Interpolated concentration of native TRAIL was measured in duplicate at different sample concentrations and interpolated from the TRAIL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean concentration was determined to be 260 pg/mL in 786-0 cell supernanant. There was no detectable signal in Hela cell supernatant.
HA722944_4.png Fig4: Interpolated concentrations of spiked TRAIL in cell culture media samples.

The concentrations of TRAIL were measured in duplicates, interpolated from the TRAIL standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.