Human NCAM1 Recombinant Rabbit Monoclonal Antibody [PSH08-08]
cat.: HA722947
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Cynomolgus monkey
Applications: ELISA(Det), ELISA(Cap)
Clonality: Monoclonal
Clone number: PSH08-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Human NCAM1 protein aa 20-718 (HA210820).
Positive control: Recombinant Human NCAM1 protein (HA210820).
Subcellular location: Cell membrane.
Recommended Dilutions:
  ELISA(Det)
  ELISA(Cap)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-07] to Human NCAM1 antibody (Capture) (HA722946) and recombinant Human NCAM1 protein as the standard (HA210820). The reference range value is 74-6,000 pg/mL.
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-09] to Human NCAM1 antibody (Detector) (HA722949) and recombinant Human NCAM1 protein as the standard (HA210820). The reference range value is 74-6,000 pg/mL.
Uniprot #: SwissProt: P13591 Human
Alternative names: antigen MSK39 identified by monoclonal 5.1H11 antigen recognized by monoclonal 5.1H11 CD56 cell adhesion molecule, neural, 1 MSK 39 MSK39 N-CAM-1 NCAM 1 NCAM NCAM C NCAM-1 NCAM1 NCAM1_HUMAN NCAMC Neural cell adhesion molecule 1 Neural cell adhesion molecule NCAM OTTHUMP00000235666
Images
HA722947_1.png Fig1: Sandwich ELISA analysis of Human NCAM1 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722946) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human NCAM1 protein (HA210820) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA722947, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA722947_2.png Fig2: Interpolated concentrations of native NCAM1 in human samples.

Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 320 ng/mL in human serum.

HA722947_3.png Fig3: Interpolated concentrations of native NCAM1 in human cell culture supernatant samples.

Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 4621 pg/mL in SH-SY5Y cell culture supernatant samples.
HA722947_4.png Fig4: Interpolated concentrations of spiked NCAM1 in cell culture media samples.

The concentrations of NCAM1 were measured in duplicates, interpolated from the NCAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA722947_5.png Fig5: Sandwich ELISA analysis of Human NCAM1 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722947) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human NCAM1 protein (HA210820) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA722949, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA722947_6.png Fig6: Interpolated concentrations of native NCAM1 in human samples.

Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 374 ng/mL in human serum.
HA722947_7.png Fig7: Interpolated concentrations of native NCAM1 in human cell culture supernatant samples.

Interpolated concentration of native NCAM1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NCAM1 concentration was determined to be 3503 pg/mL in SH-SY5Y cell culture supernatant samples.
HA722947_8.png Fig8: Interpolated concentrations of spiked NCAM1 in cell culture media samples.

The concentrations of NCAM1 were measured in duplicates, interpolated from the NCAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.