Human CTLA-4 Recombinant Rabbit Monoclonal Antibody [PSH08-14]
cat.: HA722955
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH08-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CTLA-4 aa 36-161. |
| Positive control: | Recombinant Human CTLA-4 protein (HA210885). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-13] to Human CTLA-4 antibody (Capture) (HA722954) and recombinant Human CTLA-4 protein as the standard (HA210885). The reference range value is 8.2-2,000 pg/ml. |
| Uniprot #: | SwissProt: P16410 Human |
| Alternative names: | ALPS5 CD CD 152 CD152 CD152 antigen CD152 isoform Celiac disease 3 CELIAC3 CTLA 4 CTLA-4 CTLA4 CTLA4_HUMAN Cytotoxic T cell associated 4 Cytotoxic T lymphocyte antigen 4 Cytotoxic T lymphocyte associated 4 Cytotoxic T lymphocyte associated 4, soluble isoform, included Cytotoxic T lymphocyte associated antigen 4 Cytotoxic T lymphocyte associated antigen 4 short spliced form Cytotoxic T lymphocyte associated protein 4 Cytotoxic T lymphocyte associated serine esterase 4 Cytotoxic T lymphocyte protein 4 Cytotoxic T-lymphocyte protein 4 Cytotoxic T-lymphocyte-associated antigen 4 GRD4 GSE ICOS IDDM12 insulin-dependent diabetes mellitus 12 Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 OTTHUMP00000216623 |
Images
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Fig1:
Sandwich ELISA analysis of human CTLA-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722954) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant Human CTLA-4 protein (HA210885) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722954, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of spiked CTLA-4 in cell culture media samples. The concentrations of CTLA-4 were measured in duplicates, interpolated from the CTLA-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.