Engrailed 1 Recombinant Rabbit Monoclonal Antibody [PSH08-17]
cat.: HA722960
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH08-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Engrailed 1 aa 1-392. |
| Positive control: | Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q05925 Human | P09065 Mouse Entrez Gene: 685360 Rat |
| Alternative names: | En 1 EN1 Engrailed 1 Engrailed Engrailed homeobox 1 Engrailed homolog 1 HME1_HUMAN Homeobox protein en-1 Homeobox protein engrailed 1 Homeobox protein engrailed-1 Hu-En-1 Mo-en.1 OTTHUMP00000162091 |
Images
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Fig1:
Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722960) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722960) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of SH-SY5Y cells labeling Engrailed 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722960, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.