HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]
cat.: HA722965
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH07-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is HHHHHHHHC. |
| Recommended Dilutions:
WB IHC-P |
1:100,000 1:50,000 |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
Western blot analysis of 6X His tag on different lysates with Rabbit anti-6X His tag antibody (HA722965) at 1/20,000 dilution. Lane 1: 293T transfected with His-tagged empty control cell lysate Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722965) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged ACAT2 (N-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722965) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged Histon H3.1 (C-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722965) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.