Rubicon / Baron Recombinant Rabbit Monoclonal Antibody [PSH08-25]
cat.: HA722969
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH08-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Rubicon aa 1-350. |
| Positive control: | Raji cell lysate, A549 cell lysate, NCI-H1299 cell lysate, HeLa cell lysate, 293T cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate. |
| Subcellular location: | Late endosome, Lysosome, Early endosome. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q92622 Human | Q80U62 Mouse Entrez Gene: 303885 Rat |
| Alternative names: | Baron Beclin 1 associated RUN domain containing protein Beclin-1 associated RUN domain containing protein hypothetical protein LOC9711 Kiaa0226 OTTHUMP00000208021 OTTHUMP00000208023 OTTHUMP00000208024 RUBIC_HUMAN Rubicon RUN domain and cysteine rich domain containing, Beclin 1 interacting protein Run domain Beclin 1 interacting and cystein rich containing protein Run domain Beclin-1 interacting and cysteine-rich containing protein RUN domain protein as Beclin 1 interacting and cysteine rich containing |
Images
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Fig1:
Western blot analysis of Rubicon / Baron on different lysates with Rabbit anti-Rubicon / Baron antibody (HA722969) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: A549 cell lysate Lane 3: NCI-H1299 cell lysate Lane 4: HeLa cell lysate Lane 5: 293T cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: Mouse spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 109 kDa Observed band size: 109 kDa Exposure time: Lane 1: 18 seconds; Lane 2-10: 1 minute 16 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722969) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.