Biotin Conjugated Mouse CCL3 Recombinant Rabbit Monoclonal Antibody [PSH08-40]
cat.: HA722984B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH08-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CCL3 protein aa 24-92 (HA210790). |
| Positive control: | Recombinant Mouse CCL3 protein (HA210790). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-39] to Mouse CCL3 antibody (Capture) (HA722982) and recombinant Mouse CCL3 protein (HA210790) as the standard. The reference range value is 15.6-500 pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10855 Mouse |
| Alternative names: | C C motif chemokine 3 CCL 3 CCL3 CCL3_HUMAN Chemokine (C C motif) ligand 3 Chemokine C C motif ligand 3 Chemokine ligand 3 G0/G1 switch regulatory protein 19 1 G0/G1 switch regulatory protein 19-1 G0S19 1 G0S19 1 protein Heparin binding chemotaxis protein L2G25B LD78 alpha LD78-alpha(4-69) LD78alpha Macrophage inflammatory protein 1 alpha Macrophage inflammatory protein 1-alpha macrophage inflammatory protein 1a MIP 1 alpha MIP 1A MIP-1-alpha MIP-1-alpha(4-69) MIP1 alpha MIP1A PAT 464.1 SCYA 3 SCYA3 SIS alpha SIS beta SIS-beta Small inducible cytokine A3 small inducible cytokine A3 (homologous to mouse Mip-1a) Small-inducible cytokine A3 Tonsillar lymphocyte LD78 alpha protein |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CCL3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722982) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 protein (HA210790) starting from 500 pg/ml to 0 pg/ml and detect antibody (HA722983, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.