Human CD63 Recombinant Rabbit Monoclonal Antibody [PSH08-44]
cat.: HA722989
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH08-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD63 aa 103-203 (HA210764). |
| Positive control: | Recombinant Human CD63 Protein (HA210764). |
| Subcellular location: | Cell membrane. Endosome. Lysosome. Membrane. Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-43] to Human CD63 antibody (Capture) (HA722988) and recombinant Human CD63 protein (HA210764) as the standard. The reference range value is 15.6-2,000 pg/ml. |
| Uniprot #: | SwissProt: P08962 Human |
| Alternative names: | Lysosomal associated membrane protein 3 CD 63 CD63 CD63 antigen (melanoma 1 antigen) CD63 antigen CD63 antigen melanoma 1 antigen CD63 molecule CD63_HUMAN gp55 Granulophysin LAMP 3 LAMP-3 LAMP3 LIMP Lysosomal-associated membrane protein 3 Lysosome associated membrane glycoprotein 3 Lysosome-associated membrane glycoprotein 3 Mast cell antigen AD1 ME491 Melanoma 1 antigen Melanoma associated antigen ME491 Melanoma associated antigen MLA1 Melanoma-associated antigen ME491 MGC72893 MLA 1 MLA1 NGA Ocular melanoma associated antigen Ocular melanoma-associated antigen OMA81H PTLGP40 Tetraspanin 30 Tetraspanin-30 Tspan 30 Tspan-30 TSPAN30 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD63 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722988) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD63 Protein (HA210764) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722989, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.