CGRP Recombinant Rabbit Monoclonal Antibody [PSH08-45]
cat.: HA722991
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH08-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within rat CGRP aa 1-128. |
| Positive control: | TT cell lysate, human brain tissue, mouse spinal cord tissue, rat spinal cord tissue, TT. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IHC-Fr |
1:2,000 1:100 1:200-1:1,000 1:10,000 1:500 |
| Uniprot #: | SwissProt: P06881 Human | P10092 Human | P70160 Mouse | Q99JA0 Mouse | P01256 Rat | P01257 Rat |
| Alternative names: | Calcitonin/calcitonin related polypeptide alpha Alpha type CGRP Beta type CGRP CALC1 CALC2 CALCA CALCB Calcitonin 1 Calcitonin 2 Calcitonin gene related peptide I Calcitonin gene related peptide II Calcitonin related polypeptide alpha Calcitonin related polypeptide beta CGRP CGRP I CGRP II CGRP1 CGRP2 CT Katacalcin KC |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Colon Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Western blot analysis of CGRP on different lysates with Rabbit anti-CGRP antibody (HA722991) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 10 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722991) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Western blot analysis of CGRP on different lysates with Rabbit anti-CGRP antibody (HA722991) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with rat CGRP cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722991) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CGRP antibody (HA722991) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722991) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rabbit anti-CGRP antibody (HA722991) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722991) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-CGRP antibody (HA722991) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722991) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunocytochemistry analysis of TT (positive) and HeLa (negative) labeling CGRP with Rabbit anti-CGRP antibody (HA722991) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CGRP antibody (HA722991) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig9:
Flow cytometric analysis of HeLa (left, negative) and TT (right, positive) cells labeling CGRP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722991, 1/10,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.