NK1.1 Recombinant Rabbit Monoclonal Antibody [PSH08-47]
cat.: HA722993
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH08-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse NK1.1 aa 67-223. |
| Positive control: | C57BL/6 mouse thymus tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P27814 Mouse |
| Alternative names: | CD161 antigen like family member C CD161 antigen-like family member C CD161c Killer cell lectin-like receptor subfamily B member 1C Klrb1c Klrb1f KLRBC_MOUSE Ly-55c Ly55c Lymphocyte antigen 55c Lymphocyte antigen 59 Natural killer cell surface protein P1-40 NK1.1 NKR P1.9 NKR P1C NKR-P1 40 NKR-P1.9 NKR-P1C NKRP1 |
Images
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Fig1:
Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody (HA722993) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722993) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody (HA722993) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722993) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.