DDX4 Recombinant Rabbit Monoclonal Antibody [PSH08-54]
cat.: HA723000
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH08-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DDX4 aa 201-724. |
| Positive control: | Mouse testis tissue lysate, Rat testis tissue lysate, human testis tissue, mouse testis tissue, mouse ovary tissue. |
| Subcellular location: | Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:2,000 1:1,000-1:5,000 1:500 |
| Uniprot #: | SwissProt: Q9NQI0 Human | Q61496 Mouse | Q64060 Rat |
| Alternative names: | DDX 4 Ddx4 DDX4_HUMAN DEAD (Asp Glu Ala Asp) box polypeptide 4 DEAD box protein 4 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 MVH Probable ATP dependent RNA helicase DDX4 Probable ATP-dependent RNA helicase DDX4 VASA Vasa homolog |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse testis tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723000, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Western blot analysis of DDX4 on different lysates with Rabbit anti-DDX4 antibody (HA723000) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Mouse spleen tissue lysate (negative) Lane 3: Mouse brain tissue lysate (negative) Lane 4: Rat testis tissue lysate Lane 5: Rat spleen tissue lysate (negative) Lane 6: Rat brain tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723000) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723000) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723000) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-DDX4 antibody (HA723000) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723000) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.