EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55]
cat.: HA723001
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH08-55
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 49 kDa
Isotype: IgG
Immunogen: Recombinant protein within human EXOSC9 aa 1-350.
Positive control: 293T cell lysate, HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate.
Subcellular location: Cytoplasm, Nucleus, nucleolus, nucleoplasm.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q06265 Human | Q9JHI7 Mouse | Q4QR75 Rat
Alternative names: Autoantigen PM/Scl 1 EXOS9_HUMAN EXOSC 9 EXOSC9 Exosome complex component RRP45 Exosome complex exonuclease RRP45 Exosome component 9 p5 p6 P75 polymyositis scleroderma overlap syndrome associated autoantigen P75 polymyositis-scleroderma overlap syndrome-associated autoantigen PM/Scl 75 PM/Scl-75 PMSCL autoantigen 75kD PMSCL1 Polymyositis/scleroderma autoantigen 1 75kDa Polymyositis/scleroderma autoantigen 1 Polymyositis/scleroderma autoantigen 75 kDa RRP45 Rrp45p
Images
HA723001_1.jpg Fig1: Western blot analysis of EXOSC9 on different lysates with Rabbit anti-EXOSC9 antibody (HA723001) at 1/2,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 49 kDa
Observed band size: 70 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.