Human CD137 Recombinant Rabbit Monoclonal Antibody [PSH08-63]
cat.: HA723012
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH08-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD137 aa 24-186 (HA210839). |
| Positive control: | Recombinant Human CD137 protein (HA210839). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-64] to Human CD137 antibody (Detector) (HA723013) and Recombinant Human CD137 protein (HA210839) as the standard. The reference range value is 32.9-8,000 pg/ml. |
| Uniprot #: | SwissProt: Q07011 Human |
| Alternative names: | 4 1BB 4 1BB ligand receptor 4-1BB ligand receptor 4-1BB Ligand Receptor T Cell 4-1BB, mouse, homolog of Antigen 4-1BB Homolog CD 137 CD137 CD137 antigen CDw137 HLDA VI Homolog of mouse 4 1BB ILA induced by lymphocyte activation (ILA) Induced by lymphocyte activation Interleukin activated receptor homolog of mouse Ly63 Ly63, mouse, homolog of MGC2172 OTTHUMP00000044294 Receptor protein 4 1BB T cell antigen 4 1BB homolog T cell antigen ILA T-cell antigen 4-1BB homolog T-cell antigen ILA TNF receptor superfamily member 9 TNFRSF9 TNR9_HUMAN Tumor necrosis factor receptor superfamily member 9 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD137 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723012) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD137 protein (HA210839) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723013, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD137 in HDLM-2, Jurkat and MOLT-4 extract samples based on a 2,000 µg/ml extract load. The concentrations of CD137 were measured in duplicates, interpolated from the CD137 standard curve and corrected for sample dilution. Undiluted samples are HDLM-2 extract 100%, Jurkat extract 100% and HOLT-4 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD137 concentration was determined to be 269.6 pg/ml in HDLM-2 extract and undetectable in Jurkat and MOLT-4 extract. |
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Fig3:
Interpolated concentrations of spiked CD137 in human cell culture media samples. The concentrations of CD137 were measured in duplicates, interpolated from the CD137 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.