Human CCL25 Recombinant Rabbit Monoclonal Antibody [PSH08-67]
cat.: HA723017
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH08-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL25 aa 24-150 (HA211000). |
| Positive control: | Recombinant Human CCL25 protein (HA211000). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-65] to Human CCL25 antibody (Capture) (HA723015) and recombinant Human CCL25 protein (HA211000) as the standard. The reference range value is 20.6-5,000 pg/ml. |
| Uniprot #: | SwissProt: O15444 Human |
| Alternative names: | A130072A22Rik C-C motif chemokine 25 CCL 25 CCL25 CCL25_HUMAN Chemokine (C C motif) ligand 25 Chemokine (CC motif) ligand 25 Chemokine C C motif ligand 25 Chemokine CC motif ligand 25 Chemokine TECK Ck beta 15 Ckb 15 Ckb15 MGC125074 MGC150327 SCY A25 SCYA 25 SCYA25 Small inducible cytokine A25 Small inducible cytokine A25 isoform 1 precursor Small inducible cytokine A25 isoform 2 precursor Small inducible cytokine subfamily A (Cys Cys) member 25 Small inducible cytokine subfamily A Cys Cys member 25 Small inducible cytokine subfamily A, member 25 Small-inducible cytokine A25 TECK TECKvar Thymus expressed chemokine Thymus-expressed chemokine |
Images
|
Fig1:
Sandwich ELISA analysis of Human CCL25 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723015) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL25 protein (HA211000) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA723017, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of spiked TECK in cell culture media samples. The concentrations of TECK were measured in duplicates, interpolated from the TECK standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.