LIM Kinase 1 Recombinant Rabbit Monoclonal Antibody [PSH08-68]
cat.: HA723020
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH08-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 1-350. |
| Positive control: | SH-SY5Y cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human testis tissue, mouse brain tissue, rat brain tissue, A431. |
| Subcellular location: | Cytoplasm, Nucleus, cytoskeleton, Cell projection, lamellipodium. |
| Recommended Dilutions:
WB IHC-P FC |
1:4,000 1:200-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P53667 Human | P53668 Mouse | P53669 Rat |
| Alternative names: | EC 2.7.1.37 LIM domain containing protein kinase LIM domain kinase 1 LIM kinase LIM motif containing protein kinase LIMK 1 LIMK LIMK-1 limk1 LIMK1_HUMAN |
Images
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Fig1:
Western blot analysis of LIM Kinase 1 on different lysates with Rabbit anti-LIM Kinase 1 antibody (HA723020) at 1/4,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: U-87 MG cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: COS-1 cell lysate Lane 5: Mouse liver tissue lysate (negative) Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723020) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LIM Kinase 1 antibody (HA723020) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723020) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LIM Kinase 1 antibody (HA723020) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723020) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LIM Kinase 1 antibody (HA723020) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723020) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of A431 cells labeling LIM Kinase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723020, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.