PINK1 Recombinant Rabbit Monoclonal Antibody [PSH08-69]
cat.: HA723021
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH08-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PINK1 aa 94-581 / 581. |
| Positive control: | HeLa treated with 10μM CCCP for 24 hours cell lysate, MDA-MB-231 cell lysate, human kidney tissue, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Mitochondrion outer membrane, Mitochondrion inner membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:2,000 1:50-1:1,000 1:500 |
| Uniprot #: | SwissProt: Q9BXM7 Human | Q99MQ3 Mouse | B5DFG1 Rat |
| Alternative names: | BRPK FLJ27236 mitochondrial PARK 6 PARK6 Phosphatase and Tensin Homolog PINK 1 PINK1 PINK1_HUMAN Protein kinase BRPK PTEN induced putative kinase 1 PTEN induced putative kinase protein 1 PTEN-induced putative kinase protein 1 Serine/threonine kinase PINK1 mitochondrial Serine/threonine protein kinase PINK1 mitochondrial Serine/threonine-protein kinase PINK1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig3:
Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (HA723021) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate Lane 3: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723021) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.