HA723021

PINK1 Recombinant Rabbit Monoclonal Antibody [PSH08-69]

cat.: HA723021

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IHC-Fr
Clonality:	Monoclonal
Clone number:	PSH08-69

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 63 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human PINK1 aa 94-581 / 581.
Positive control:	HeLa treated with 10μM CCCP for 24 hours cell lysate, MDA-MB-231 cell lysate, human kidney tissue, human brain tissue, mouse brain tissue, rat brain tissue.
Subcellular location:	Mitochondrion outer membrane, Mitochondrion inner membrane, Cytoplasm, cytosol.
Recommended Dilutions: WB IHC-P IHC-Fr	1:2,000 1:50-1:1,000 1:500
Uniprot #:	SwissProt: Q9BXM7 Human \| Q99MQ3 Mouse \| B5DFG1 Rat
Alternative names:	BRPK FLJ27236 mitochondrial PARK 6 PARK6 Phosphatase and Tensin Homolog PINK 1 PINK1 PINK1_HUMAN Protein kinase BRPK PTEN induced putative kinase 1 PTEN induced putative kinase protein 1 PTEN-induced putative kinase protein 1 Serine/threonine kinase PINK1 mitochondrial Serine/threonine protein kinase PINK1 mitochondrial Serine/threonine-protein kinase PINK1

Images

	Fig1: Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723021, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig2: Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723021, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig3: Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (HA723021) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate Lane 3: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723021) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.