HA723025

MAP2 Recombinant Rabbit Monoclonal Antibody [PSH08-73]

cat.: HA723025

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey
Applications:	WB, IHC-P, IHC-Fr, IF-Cell, mIHC, VIVIT, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH08-73

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 200 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human MAP2 aa 1-600.
Positive control:	Mouse primary neural cells, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.
Subcellular location:	Cytoplasm, cytoskeleton, Cell projection, dendrite.
Recommended Dilutions: WB IHC-P IHC-Fr IF-Cell mIHC IF-Tissue	1:2,000-1:5,000 1:500-1:8,000 1:500 1:500 1:20,000 1:1,000
Uniprot #:	SwissProt: P11137 Human \| P20357 Mouse \| P15146 Rat
Alternative names:	DKFZp686I2148 MAP 2 MAP dendrite specific MAP-2 MAP2 MAP2A MAP2B MAP2C Microtubule associated protein 2 Microtubule-associated protein 2 MTAP2_HUMAN

Images

	Fig1: mIHC analysis of mouse brain tissue (Frozen section) with MAP2 (HA723025, 1/20,000) and antibody VGLUT1 (HA601450, 1/2,000). The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
	Fig2: Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (MAP2, HA723025, red); 1/1,000 (Iba1, RT1316, green) Antigen retrieval: Not required
	Fig3: Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (MAP2, HA723025, red); 1/1,000 (Iba1, RT1316, green) Antigen retrieval: Not required
	Fig4: Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: MAP2 (HA723025, 1/500, red); DCX (HA601398, 1/500, green) Antigen retrieval: Not required

	Fig5: Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
	Fig6: Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: Mouse primary neural cells Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA723025, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
	Fig7: Western blot analysis of MAP2 on different lysates with Rabbit anti-MAP2 antibody (HA723025) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse kidney tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 200 kDa Observed band size: 150-300 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723025) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig8: Western blot analysis of MAP2 on different lysates with Rabbit anti-MAP2 antibody (HA723025) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Rat brain tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 200 kDa
Observed band size: 150-300 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723025) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig9: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAP2 antibody (HA723025) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723025) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAP2 antibody (HA723025) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723025) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig11: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAP2 antibody (HA723025) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723025) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig12: Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA723025, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.