DNA polymerase mu Recombinant Rabbit Monoclonal Antibody [PSH08-75]
cat.: HA723027
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH08-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DNA polymerase mu aa 1-494. |
| Positive control: | HeLa cell lysate, Daudi cell lysate, HEK-293 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9NP87 Human |
| Alternative names: | DNA-directed DNA polymerase mu DNA-directed DNA/RNA polymerase mu DPOLM_HUMAN FLJ35482 Pol iota Pol Mu Polm POLM protein Polymerase (DNA directed) mu Polymerase DNA directed mu Tdt N Tdt-N TdtN Terminal transferase |
Images
|
Fig1:
Western blot analysis of DNA polymerase mu on different lysates with Rabbit anti-DNA polymerase mu antibody (HA723027) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 52 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723027) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.