LIFR Recombinant Rabbit Monoclonal Antibody [PSH08-76]
cat.: HA723028
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH08-76
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 124 kDa
Isotype: IgG
Immunogen: Recombinant protein within human LIFR aa 1-850.
Positive control: HeLa cell lysate, PANC-1 cell lysate, MDA-MB-231 cell lysate, HCT 116 cell lysate, RD cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
1:2,000
Uniprot #: SwissProt: P42702 Human
Alternative names: CD118 CD118 antigen FLJ98106 FLJ99923 Leukemia inhibitory factor receptor alpha Leukemia inhibitory factor receptor LIF R LIF receptor LIF-R Lifr LIFR_HUMAN SJS2 STWS SWS
Images
HA723028_1.jpg Fig1: Western blot analysis of LIFR on different lysates with Rabbit anti-LIFR antibody (HA723028) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: T-47D cell lysate (negative)
Lane 4: MDA-MB-231 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: RD cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 124 kDa
Observed band size: 180 kDa

Exposure time: 46 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.