Alpha-Synuclein Recombinant Rabbit Monoclonal Antibody [PSH08-83]
cat.: HA723035
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH08-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Alpha-Synuclein aa 1-140. |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus, Synapse, Secreted, Cell projection, axon. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:2,000-1:5,000 1:5,000 1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: P37840 Human | O55042 Mouse | P37377 Rat |
| Alternative names: | Alpha synuclein Alpha-synuclein Alpha-synuclein, isoform NACP140 alphaSYN MGC105443 MGC110988 MGC127560 MGC64356 NACP Non A beta component of AD amyloid Non A4 component of amyloid Non A4 component of amyloid precursor Non-A beta component of AD amyloid Non-A-beta component of alzheimers disease amyloid , precursor of Non-A4 component of amyloid precursor Non-A4 component of amyloid, precursor of OTTHUMP00000218549 OTTHUMP00000218551 OTTHUMP00000218552 OTTHUMP00000218553 OTTHUMP00000218554 PARK 1 PARK 4 PARK1 PARK4 Parkinson disease (autosomal dominant, Lewy body) 4 Parkinson disease familial 1 SNCA Snca synuclein Snca synuclein, alpha (non A4 component of amyloid precursor) SYN Synuclein alpha Synuclein alpha 140 Synuclein, alpha (non A4 component of amyloid precursor) SYUA_HUMAN |
Images
|
Fig1:
Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723035, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723035, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with Alpha-Synuclein cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 14 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723035) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/5,000 dilution. Lane 1: Human SCNA recombinant protein, 30ng/Lane Lane 2: Human SCNB recombinant protein, 30ng/Lane Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723035) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723035) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (low expression) with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723035) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.