TREX1 Recombinant Rabbit Monoclonal Antibody [PSH08-84]
cat.: HA723036
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH08-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse TREX1 aa 1-280. |
| Positive control: | RAW264.7 cell lysate, CTLL-2 cell lysate, J774A.1 cell lysate, RAW264.7. |
| Subcellular location: | Nucleus, Cytoplasm, cytosol, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB FC IP |
1:2,000-1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q91XB0 Mouse |
| Alternative names: | 3' 5' exonuclease TREX1 3' repair exonuclease 1 AGS1 AGS5 CRV Deoxyribonuclease III, dnaQ/mutD (E. coli) like DKFZp434J0310 DNase III DRN3 HERNS Three prime repair exonuclease 1 TREX1 |
Images
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Fig1:
Western blot analysis of TREX1 on different lysates with Rabbit anti-TREX1 antibody (HA723036) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: CTLL-2 cell lysate Lane 3: J774A.1 cell lysate Lane 4: Neuro-2a cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: Lane 1: 25 seconds; Lane 2-4: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723036) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of Neuro-2a (left, negative) and RAW264.7 (right, positive) cells labeling TREX1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723036, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig3:
TREX1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA723036 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA723036 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA723036 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA723036 in RAW264.7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 16 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.