AMPK alpha 1/2 Recombinant Rabbit Monoclonal Antibody [PSH08-86]
cat.: HA723038
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH08-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64/62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AMPK alpha 2 aa 1-400. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q13131 Human | P54646 Human | Q5EG47 Mouse | Q8BRK8 Mouse | P54645 Rat | Q09137 Rat |
| Alternative names: | 5 AMP activated protein kinase alpha 1catalytic subunit 5 AMP activated protein kinase catalytic alpha 1 chain 5' AMP activated protein kinase catalytic subunit alpha 1 5'-AMP-activated protein kinase catalytic subunit alpha-1 AAPK1 AAPK1_HUMAN ACACA kinase acetyl CoA carboxylase kinase AI194361 AI450832 AL024255 AMP -activate kinase alpha 1 subunit AMP-activate kinase alpha 1 subunit AMP-activated protein kinase, catalytic, alpha -1 AMPK 1 AMPK alpha 1 AMPK alpha 1 chain AMPK AMPK subunit alpha 1 AMPK subunit alpha-1 AMPK1 AMPKa1 AMPKalpha1 C130083N04Rik cb116 EC 2.7.11.1 HMG CoA reductase kinase HMGCR kinase hormone sensitive lipase kinase Hydroxymethylglutaryl CoA reductase kinase im:7154392 kinase AMPK alpha1 MGC33776 MGC57364 OTTHUMP00000161795 OTTHUMP00000161796 PRKAA 1 PRKAA1 Protein kinase AMP activated alpha 1 catalytic subunit SNF1-like protein AMPK SNF1A Tau protein kinase PRKAA1 wu:fa94c10 ...... |
Images
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Fig1:
Western blot analysis of AMPK alpha 1/2 on different lysates with Rabbit anti-AMPK alpha 1/2 antibody (HA723038) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Predicted band size: 64/62 kDa Observed band size: 60 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723038) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-AMPK alpha 1/2 antibody (HA723038) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723038) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-AMPK alpha 1/2 antibody (HA723038) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723038) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-AMPK alpha 1/2 antibody (HA723038) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723038) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-AMPK alpha 1/2 antibody (HA723038) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723038) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of AMPK alpha 1/2 on different lysates with Rabbit anti-AMPK alpha 1/2 antibody (HA723038) at 1/2,000 dilution. Lane 1: His-tagged Human AMPK alpha 1 recombinant protein Lane 2: His-tagged Human AMPK alpha 2 recombinant protein Lysates/proteins at 30 ng/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723038) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.