Mouse/Rat Leptin Recombinant Rabbit Monoclonal Antibody [PSH08-95]
cat.: HA723047
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH08-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Leptin aa 22-167. |
| Positive control: | Recombinant Mouse Leptin protein (HA210898). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-94] to Mouse/Rat Leptin antibody (Capture) (HA723046) and recombinant Mouse/Rat Leptin protein (HA210898) as the standard. The reference range value is 31-4,000 pg/ml. |
| Uniprot #: | SwissProt: P41160 Mouse | P50596 Rat |
| Alternative names: | FLJ94114 LEP LEP_HUMAN LEPD Leptin (murine obesity homolog) Leptin (obesity homolog, mouse) Leptin Leptin Murine Obesity Homolog Leptin Precursor Obesity Factor OB Obese protein Obese, mouse, homolog of Obesity Obesity factor Obesity homolog mouse Obesity Murine Homolog Leptin OBS OTTHUMP00000212285 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse/Rat leptin matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723046) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Leptin protein (HA210898) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723047, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native leptin in mouse serum and rat serum samples. The concentrations of leptin were interpolated from the leptin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 25% and rat serum 25%. The mean leptin concentration was determined to be 9,628 pg/ml in mouse serum and 2,283 pg/ml in rat serum. |
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Fig3:
Interpolated concentrations of spiked leptin in mouse cell culture media samples. The concentrations of leptin were measured in duplicates, interpolated from the leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.