Phospho-MYPT1 (T696) Recombinant Rabbit Monoclonal Antibody [PSH08-96]
cat.: HA723049
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH08-96 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 115 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr696 of Human MYPT1. |
| Positive control: | HeLa cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, stress fiber. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O14974 Human | Q9DBR7 Mouse | Q10728 Rat |
| Alternative names: | M130 MBS Myosin binding subunit Myosin phosphatase target subunit 1 Myosin phosphatase targeting subunit 1 Myosin phosphatase-targeting subunit 1 MYPT1 MYPT1_HUMAN PPP1R12A Protein phosphatase 1 regulatory inhibitor subunit 12A Protein phosphatase 1 regulatory subunit 12A Protein phosphatase 1, regulatory (inhibitor) subunit 12A Protein phosphatase myosin binding subunit Protein phosphatase myosin-binding subunit |
Images
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Fig1:
Western blot analysis of Phospho-MYPT1 (T696) on different lysates with Rabbit anti-Phospho-MYPT1 (T696) antibody (HA723049) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM Y-27632 for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 140 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723049) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.